HLA B*5701 / B27
Approximately 5-9% of patients treated with abacavir, a nucleoside analogue with antiviral activity agianst HIV, develop a hypersenitivity reaction (HSR). This HSR is characterized by a multisystem involvement who proved fatal in some cases. Several studies have shown a strong predictive association between for HLA-B*5701 carriage and abacavir HSR. The following kit is a multiplex SSP-PCR for typing HLA*B5701. In each PCR reaction are simultaneously amplified the HLA- *5701 allele and a conserved region of β- globin human gene as internal control, guaranteeing reliable results. The analysis of amplified product is performed through gel agarose electrophoresis or allele specific real time PCR (AS-PCR). Human Leukocyte Antigen (HLA) B27 (subtypes B*2701-2759) is an HLA class I surface antigen that is encoded in the B locus in the major histocompatibility complex (MHC) on the short arm of chromosome 6. In HLA-B27 antigen a strong association with ankylosing spondylitis (AS) and spondyloarthropathies (SpA) was found. The following kit detects HLA-B27 allele (subtypes B*2701-2759) using As internal control the gene SYPL2 (synaptophysin-like 2) by real time PCR.
| Ref. | Product | Technology | Size | Regulatory | Additional Info |
|---|---|---|---|---|---|
| AH.01 | B*5701 Kit 1 | Agarose Gel Electrophoresis Kit | 40 |  | Allelic spcific primer PCR |
| AH.01RQ | B*5701 Kit 1 - RQ | Real Time PCR Kit | 40 | | Allelic spcific primer PCR |
| K.B27.RQ | Spondylitis HLA-B27 RQ | Real Time PCR Kit | 50 | | Allelic spcific primer PCR |