HLA B*5701 / B27

Approximately 5-9% of patients treated with abacavir, a nucleoside analogue with antiviral activity agianst HIV, develop a hypersenitivity reaction (HSR). This HSR is characterized by a multisystem involvement who proved fatal in some cases. Several studies have shown a strong predictive association between for HLA-B*5701 carriage and abacavir HSR. The following kit is a multiplex SSP-PCR for typing HLA*B5701. In each PCR reaction are simultaneously amplified the HLA- *5701 allele and a conserved region of β- globin human gene as internal control, guaranteeing reliable results. The analysis of amplified product is performed through gel agarose electrophoresis or allele specific real time PCR (AS-PCR). Human Leukocyte Antigen (HLA) B27 (subtypes B*2701-2759) is an HLA class I surface antigen that is encoded in the B locus in the major histocompatibility complex (MHC) on the short arm of chromosome 6. In HLA-B27 antigen a strong association with ankylosing spondylitis (AS) and spondyloarthropathies (SpA) was found. The following kit detects HLA-B27 allele (subtypes B*2701-2759) using As internal control the gene SYPL2 (synaptophysin-like 2) by real time PCR.

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HLA B*5701 / B27
Ref.ProductTechnology SizeRegulatoryAdditional Info
AH.01B*5701 Kit 1

Agarose Gel Electrophoresis Kit

40AH.01

Allelic spcific primer PCR

AH.01RQB*5701 Kit 1 - RQ

Real Time PCR Kit

40AH.01RQ

Allelic spcific primer PCR

K.B27.RQSpondylitis HLA-B27 RQ

Real Time PCR Kit

50K.B27.RQ

Allelic spcific primer PCR