Pharmacogenetics

Pharmacogenetics

IL28B - (pdf info)

It has been recently demonstrated by studies of Genome Wide Association (GWA) that the single nucleotide polymorphisms (SNPs) rs12979860 and rs8099917 in the gene interleukin-28B (IL28B) coding for IFN-λ 3 are closely associated with virologic response for the treatment PegIFN-alpha-2a / RBV.
Experteam offers a complete system for allelic discrimination (genotyping) of rs12979860 and rs8099917 in the interleukin-28B (IL28B) gene.

HLA B*5701 / B27 - (pdf info)

Approximately 5-9% of patients treated with abacavir, a nucleoside analogue with antiviral activity agianst HIV, develop a hypersenitivity reaction (HSR). This HSR is characterized by a multisystem involvement who proved fatal in some cases. Several studies have shown a strong predictive association between for HLA-B*5701 carriage and abacavir HSR. The following kit is a multiplex SSP-PCR for typing HLA*B5701. In each PCR reaction are simultaneously amplified the HLA- *5701 allele and a conserved region of β- globin human gene as internal control, guaranteeing reliable results. The analysis of amplified product is performed through gel agarose electrophoresis or allele specific real time PCR (AS-PCR).
Human Leukocyte Antigen (HLA) B27 (subtypes B*2701-2759) is an HLA class I surface antigen that is encoded in the B locus in the major histocompatibility complex (MHC) on the short arm of chromosome 6. In HLA-B27 antigen a strong association with ankylosing spondylitis (AS) and spondyloarthropathies (SpA) was found.
The following kit detects HLA-B27 allele (subtypes B*2701-2759) using As internal control the gene SYPL2 (synaptophysin-like 2) by real time PCR.